Journal: bioRxiv
Article Title: Non-cell-autonomous control of gastruloid development by the lncRNA T-UCstem1 through DKK1-dependent modulation of WNT signalling
doi: 10.1101/2024.09.18.613704
Figure Lengend Snippet: A) Principal-component analysis (PCA) of the expression profiles of NT and T-UCstem1 KD-1 gastruloids, treated with 3 μM, at 120 h. B) Enrichment of top 5 biological process gene ontology terms ordered by p-value of upregulated genes in T-UCstem1 KD-1 versus Control (NT) gastruloids. C) Heatmaps of upregulated and downregulated genes related to pluripotency, A-P pattern specification and WNT signalling in T-UCstem1 KD-1 cells versus Control (NT). D) UMAP plot of the subsampled dataset with annotated clusters. E) Odd ratios (OR) of annotated cell populations, in T-UCstem1 KD-1 vs NT gastruloids. F) UMAP expression plot of Oct4, Bra, FoxA2 and Nes in T-UCstem1 KD-1 gastruloids.
Article Snippet: ESCs were seeded at low density (250 cells/cm 2 ) on gelatin-coated plates in DMEM/FBS containing a mix of three naïve pluripotency-inducing cytokines/inhibitors: Leukemia Inhibitor Factor (LIF; 1,000 u/mL, ESGRO-Millipore), a WNT signalling agonist (CHIR99021; 3 μM, Selleckchem), and a MEK inhibitor (PD0325091; 1 μM, Selleckchem).
Techniques: Expressing, Control